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Zheng et al. Cryo-electron tomography reconstructs polymer in liquid film for fab-compatible lithography, Nature Communications, September 30, 2025 Understanding protein structures at the molecular level is of paramount importance in contemporary biomedicine.1 The correlation between variations in protein structures and … Continue reading →

Fan et al. Electrospray-based sample preparation for high-resolution protein structure elucidation by cryo-EM. Clin Transl Med., August 10, 2025 Understanding protein structures at the molecular level is of paramount importance in contemporary biomedicine.1 The correlation between variations in protein structures and their … Continue reading →

Cao et al. Structural basis of endo-siRNA processing by Drosophila Dicer-2 and Loqs-PD. Nucleic Acids Research, February 8, 2025 Endogenous small interfering RNAs (endo-siRNAs or esiRNAs) originate from either elongated endogenous transcripts capable of forming complex fold-back structures or from double-stranded … Continue reading →

Zheng et al. Cryo-electron tomography reconstructs polymer in liquid film for fab-compatible lithography. Nature Communications, September 30, 2025 Liquid film is ubiquitous in nature and serves as the critical medium for the dissolution of photoresist to create nanoscale circuit patterns in lithography, … Continue reading →

Zheng et al. Self-assembled superstructure alleviates air-water interface effect in cryo-EM. Nature Communications, August 24, 2024 Cryo-electron microscopy (cryo-EM) has been widely used to reveal the structures of proteins at atomic resolution. One key challenge is that almost all proteins … Continue reading →

Yang et al. Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods, June, 2024 Addressing interfacial effects during specimen preparation in cryogenic electron microscopy remains challenging. Here we introduce ESI-cryoPrep, a specimen preparation method based on electrospray ionization in … Continue reading →

Xu et al. Graphene sandwich-based biological specimen preparation for cryo-EM analysis. PNAS, January 30, 2024 High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called … Continue reading →

Zhou et al. Structural insights into the mechanism of GTP initiation of microtubule assembly. Nature Communications, September 25, 2023 In eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP … Continue reading →

You et al. In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex. Nature, April, 2023 In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis1,2. The … Continue reading →

Zheng et al. Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nature Methods, January, 2023 Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, the homogeneity of ice thickness, … Continue reading →