Architecture and mechanism of the RISC-loading complex (RLC) in RNA interference pathways

RLC is at the core of RNA interference pathways, responsible for generating siRNAs and miRNAs and loading them into RNA- induced silencing complex for gene-silencing effects. The structure of RLC and the mechanisms of RNA substrates processed by this complex are revealed using cryo-EM.

Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, NolandCL, Nagayama K, Nogales E, Doudna JA*, and Wang HW*. (2013) Substrate-specific structural rearrangements of human Dicer. Nat. Struct. Mol. Biol., 20:662-670 (* co-corresponding authors)